Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQEKRLKAIQNKIASWIKEIESGFIDALFSKIGP--SKMLRSKLMLALLDEKTDAI-LLDKALNLCAIVEMIQTASLLHDDVIDKATMRRKLPSINALFGNFNAVMLGDVFYSKAFFELSKMGES-IAQALSNAVLRLSRGE-IEDVFVGECFNSDKQKYWRILEDKTAHFIEASLKSMAILLNKDAKMYADFGLHFGMAFQIIDDLLDITQDANTLGKPNFSDFKEGKTTLPYLLLYEKLNQHEQGLLISYFKQDSHEIIEWTKEKFKQHGII---EETLKIAQVYSKKALEAIKGENNLILEKLAQDVIYRTF 1V4H Chain:A ((15-273)) -----------KVKERIEQILSQFFPEQIMKDLPLYGKMLRVRL--SILSAKNRGVEIGEDAISSLAALELVHLASLLHDDVIDGARFRRGKETINFMYGDKAAVAAGDLVLVSAFHTVEEIGNNKLRRAFLNVIGKMSEAELIEQLSRYKPIT--KEEYLRIVEGKSGALFGLALQLPALLEGELGEDLYNLGVTIGTIYQMFDDIMDFA-GMEKIGKDGFLDLKNGVASFPLVTAMEKFPEARQ----MFENRDWSGLMSFMREK----GILKECEETLKVLVKNVIIENSWLRD------------------

General information: TITO was launched using: RESULT: Template: 1V4H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -97118 for 1993 contacts (-48.7/contact) + 2D Compatibility (PS) -26948 + (NN) -14617 + (LL) 3800 1D Compatibility (HY) -21200 + (ID) 4050 Total energy: -160133.0 ( -80.35 by residue) QMean score : 0.428

(partial model without unconserved sides chains): PDB file : Tito_1V4H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V4H-query.scw PDB file : Tito_Scwrl_1V4H.pdb: