Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLYTMKDLLAVGKEHQFAVPAFNICSFDMLKAIMEEVEANNAPVILEIHPDEIEYLGDNFVATVREYAYRSK--VPVVIHMDHGGTIKDVMRAIRNGYTSVMIDASRASYEENVALTTQVVELAHKVGVSVEAELGTIGNNGSAEGGADTIIYTDPDQAEDFVSRTGIDTLAVAIGTAHGLYPKDKKPELNMPL--LKELNKRLDIPFVLHGGS---------------------GNPDKEVSESVQYGVRKVNLSSDLKSVFFEEVRRVLVDNPAMYEPNQVYPSANEKVKDVVRHKLDILNTTGQADKY 2ISV Chain:A ((3-310)) -LCTLRQMLGEARKHKYGVGAFNVNNMEQIQGIMKAVVQLKSPVILQCSRGALKYSDMIYLKKLCEAALEKHPDIPICIHLDHGDTLESVKMAIDLGFSSVMIDASHHPFDENVRITKEVVAYAHARSVSVEAELG----------------LTEPQDAKKFVELTGVDALAVAIGTSHGA---------RLAIDRVKTISDLTGIPLVMHGSSSVPKDVKDMINKYGGKMPDAVGVPIESIVHAIGEGVCKINVDSDSRMAMTGAIRKVFVEHPEKFDPRDYLGPGRDAITEMLIPKIKAFGSAGHAGDY

General information: TITO was launched using: RESULT: Template: 2ISV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -134683 for 2324 contacts (-58.0/contact) + 2D Compatibility (PS) -29034 + (NN) -14658 + (LL) 572 1D Compatibility (HY) -25600 + (ID) 4700 Total energy: -208103.0 ( -89.55 by residue) QMean score : 0.620

(partial model without unconserved sides chains): PDB file : Tito_2ISV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ISV-query.scw PDB file : Tito_Scwrl_2ISV.pdb: