Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDFLHHILSTYASFFDWKMWGEVLTDPVSWGLIGSLVVLEGLLSADNALV------LAVMVKHLPEKQRKKALTYGLFGAYIF--------------RFIFIGL--------GMLLIKFWWIKVLG--------ALYLAWLVIKHFWIGEKE--------------EEADGMKKNSWMVRTFGIF--WATVISV-------ELMDLAFSVDSIL--------AAFAVSEKVW----------VLLIGGMLGILMMRTVAKVFLVLIDKIPELENTAFV----------LIGIIALKMAGSAFHYEM----PHSVFFIIIIAAFAVTLIIHYINKQKQVREQTAASKEE 3H5N Chain:A ((4-350)) MD---YILGRYVKIARYGSGGLVGGGGKE-QYVENLVLWENIIKTAYCFITPSSYTAALETANIPEKDFSNCFRFLKENFFIIPGEYNNSTENNRYSRNFLHYQSYGANPVLVQDKLKNAKVVILGCGGIGNHVSVILATSGIGEIILIDNDQIENTNLTRQVLFSEDDVGKNKTEVIKRELLKRNSEISVSEIALNINDYTDLHKVPEADIWVVSADHPFNLINWVNKYCVRANQPYINAGYVNDIAVFGPLYVPGKTGCYECQKKENIDHKIKLINSRFKPATFAPVNNVAAALCAADVIKFIGKYSEPLSLNKRIGIWSDEIKIHSQNMGRSPVCSVCG------

General information: TITO was launched using: RESULT: Template: 3H5N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1021 -191842 -187.90 -776.69 target 2D structure prediction score : 0.26 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -187.90 2D Compatibility (Sec. Struct. Predict.) : 0.26 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.204

(partial model without unconserved sides chains): PDB file : Tito_3H5N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3H5N-query.scw PDB file : Tito_Scwrl_3H5N.pdb: