Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTASQQQIQLARRPQGIPVHEDFRFET-----IPVPEPKQGEVLVKTLYVSVDPYMRGRM--QDTKSYVEPFALDKALSGGVIAEVVSDGNH--LKKGDIVIGNLSWQEFSAVSE-SALRKIDTSLAPASAYLGILGMTGLTAYFGLLDIGRPKEGETVVVSGAAGAVGSTVGQIAKIKGARVVGIAGSDEKIDYLKQELQFDEAINYKTADDIQKALQNACPDGVDVYFDNVGGPISDAVMNLLNEFARIPVCGAISSYNAESEADDMGPRVQSKLIKTKSLMQGFIVSDYSDRFSEGAKQLAEWLKAGKLHYEETITEGFENIPDAFLGLFKGENKGKQLIKVSDPS 4WGG Chain:A ((15-342)) -------------PEGAPKETDMELVTTGTIRLRVPEGSNA-VLLKNLYLSCDPYMRMRMTKHEEASYVDDFVPGAPITGFGVGKVV-DSSHPDFKTGDYVWGLIGWEEYSLITKPQGLFKIHHTEIPLSYYTGILGMVGLTAYVGFYDICSPKKGERVFVSAAAGAVGQIVGQFAKQFGCYVVGSAGSDEKVNLLKTKFGFDEAFNYKKEPDLTKALKRYFPEGIDIYFENVGGPMLEAVLHNMRIKGRIAACGMISQYNLEKPE---GVHNLFLIVGKRIRLEGFLVFDHYGSYPEFEEKVVQLIKEEKIKYLEDIVEGLENAPAALIGLFEGRNVGKQVVVVS---

General information: TITO was launched using: RESULT: Template: 4WGG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1795 -194537 -108.38 -611.75 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -108.38 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.565

(partial model without unconserved sides chains): PDB file : Tito_4WGG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WGG-query.scw PDB file : Tito_Scwrl_4WGG.pdb: