Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGIAGTFIFMIVIGAAIGAVTNHLAIQMLFRPYKAYYLFGKRVPFTPGLIPRRRDELAKQMGLMVVNHLLTPEGIKKRLVSDAAKTQALRVGEQLIQKL-SLSEVTVKEALEKAGMKRPEKAADAWISSWTDDKLHELFRQYGDQSLKELVPIEVQEKLEEKIPMISGYILSRSVRYFESDEGKIRLGNMIDDFLKERGMLGSMVQLFLGNSSLADRVLPELLKFLRNEETNKLLSDLLKNEWGKLREYTFNEADEKWNAKALIFSLKRRVLQAFSTAPFFNNTIGTLTVRYESELTQQMLPALLDKLLEGISSNLESVLKRLRLEEIVKEQVDQFPVERLEEMVLSISKKEFKMITYLGGLLGGIIGAIQALFVILF 3R1B Chain:A ((285-370)) -------------------------------------------------------------------------------------STTLRYGFLLMLKYPHVTERVQKEIEQVIGSHRPPALDDRAKMPYTDAVIHEI------QRLGDLIPFGVPHTVTKDT-QFRGYVIPKNTEVF--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3R1B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 174 -6589 -37.87 -77.52 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -37.87 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.248

(partial model without unconserved sides chains): PDB file : Tito_3R1B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3R1B-query.scw PDB file : Tito_Scwrl_3R1B.pdb: