Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRVEPPYDVKEALVFTQKMAQLSKALWKSIEKDWQQWLKPYDLNINEHHILWIAYQLNGASISEIAKFGVMHVSTAFNFSKKLEERGYLRFSKRLNDKRNTYVQLTEEGTEVFWSLLEEFDPTRNAVFKGSQPLYHLFGKFPEVAEMMCMIRHIYGDDFMEIFETSLTNIDNDFESVNGKLKKKAKDSAADEPAEELEPVNS 2FA5 Chain:A ((25-120)) ------------------RLSVLSNRISGNIAKVYGDR---YGMAIPEWRVITILALYPGSSASEVSDRTAMDKVAVSRAVARLLERGFIR---------RSMLALSPAGRQVYETV--------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2FA5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 320 -53926 -168.52 -619.83 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -168.52 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_2FA5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FA5-query.scw PDB file : Tito_Scwrl_2FA5.pdb: