Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMMKYEAVIIGGGPVGFMLASELAIAGVGTCVIERLEKPVPHSKALTLHPRTLELLEMRGILERFVSKG--------------------SKIPTGHFSMLDTRLDFSGLDTSCPYTLLLPQSKTEKLLEDHARSLGTEVFRGAEALAVTQNGEAVQTIFKD-RDGSVRTITSKFAVGADGAGSTVRKQAKIEFPGTDSTVTAALGDVVLLSPPPSGVLSLCTKEGGVMIVPLSPDRYRVVVISP-----YRTQTPKDVPVTEEELKADLLRIC---GTDFG-LTDPSWMSRFGNAARQAKRYRDGRIFLAGDAAHIHFPAGGQGLNVGLQDAMNLGWKLAAAIKGSAPSWLLDSYHDERHPAAEGLLRNTEAQTK----------LIDFTQAGLHLRSMMSELLAFPDVNR------------YVAGQISALDVRYEADRTMPPNRLNGARLPDMKLILSDGNSE---RLYSFLQNGTF--VLLSLRQEADDHIEVKGLRTVTASLAEPNEKLRNVHTILIRPDGHVAWAVDASAPDCSEVIQKGISRWFSVTSRV 2R0P Chain:A ((25-544)) --IETDVLILGGGPVGMALALDLAHRQVGHLVVEQTDGTITHPRVGTIGPRSMELFRRWGVAKQIRTAGWPGDHPLDAAWVTRVGGHEVYRIPLGTADTRATPEHTPEPDAICPQHWLAP------LL---AEAVGERLRTRSRLDSFEQRDDHVRATITDLRTGATRAVHARYLVACDGASSPTRKALGIDAPPRHRT--QVFRNILFRAPE---LRSLLGERAALFFFLML---LRFPLRALDGRGLYRLTVGVD-----TMDSFELVRRAVAFDTEIEVLSDSEWHL----THRVADSFSAGRVFLTGDAAHTLSPSGGFGMNTGIGSAADLGWKLAATLRGWAGPGLLATYEEERRPVAITSLEEANVNLRRTMDRELPPGLHDDGPRGERIRAAVAEKLERSGARREFDAPGIHFGHTYRSSIVCGE---------WRPSARPGARAPHAWLTPTTSTLDLFGRGFVLLSFGTTDGVEAVTRAFADRHVP---LETVTCHAPEIHALYERAH-VLVRPDGHVAWRGDHLPAELGGLVDK------------

General information: TITO was launched using: RESULT: Template: 2R0P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2359 -211612 -89.70 -474.47 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -89.70 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.398

(partial model without unconserved sides chains): PDB file : Tito_2R0P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R0P-query.scw PDB file : Tito_Scwrl_2R0P.pdb: