Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALKAQNTISGKEGRLFLDG---------------EEMAHIKTFE-ANVEKNKSEVNIMGRRM---TGHKTTGANG-TGTATFYKV-TSKFVLLMMDYVKKGSDPYFTLQAVLDDQSSGRGTERVTLYDVNFDSAKIASLDVDSEALEEEVPFTFEDFDVPEKLSDTF 2K4Q Chain:A ((8-149)) ---NPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKDLTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNEGDTRAYKIR----FPN---GT-VDVF-RGWVSSIG-KAVTAK-EVITRTVKVTNV------------

General information: TITO was launched using: RESULT: Template: 2K4Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 409 -26593 -65.02 -219.77 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -65.02 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.348

(partial model without unconserved sides chains): PDB file : Tito_2K4Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2K4Q-query.scw PDB file : Tito_Scwrl_2K4Q.pdb: