Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTCFLSIWRVVDPIYFFFSRLSLIDNDQKSVFRVRLTKYKGHHV---VLSDG---THIRKNDVLVKIHLHNIKLIRELQSIESAVRKGIIIYQKVYQSMP------LLLDYINNHKKS--EKIKGIIGITMLDKGVERLGFDV------ITPVNPFYRCFKKV--------SHVPILYLTSKPVSLRHLPNSSYLFISKEKLQKTYQKKD 4LXS Chain:A ((127-318)) ---------------------------------RFRFTTRRLTHIPANLLTDMRNLSHLELRANIEEMPSHLFDDLENLESIEFGSNKLRQMPRGIFGKMPKLKQLNLWSNQLHNLTKHDFEGATSVLGIDIHDNGIEQLPHDVFAHLTNVTDINLSANLFRSLPQGLFDHNKHLNEVRLMNNRVPLATLP--SRLFANQPELQ-------

General information: TITO was launched using: RESULT: Template: 4LXS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 534 -53876 -100.89 -382.10 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -100.89 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.165

(partial model without unconserved sides chains): PDB file : Tito_4LXS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LXS-query.scw PDB file : Tito_Scwrl_4LXS.pdb: