Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRMSLIGERFTEEEQKLLLNILINHEYAIELLSSEINDIETGTKNVDGTTYKKLVTLYDRFRFEN 2K0M Chain:A ((32-69)) ----RPGDIVSTVDGAFLVEALKRHPDATSKIGPGVRNFEVR-----------------------

General information: TITO was launched using: RESULT: Template: 2K0M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 56 -6514 -116.31 -171.41 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -116.31 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.660

(partial model without unconserved sides chains): PDB file : Tito_2K0M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2K0M-query.scw PDB file : Tito_Scwrl_2K0M.pdb: