Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFQSTEIGIDLGTANILVYSKNKGIILNEPSVVAVDTT--TKAVLAIGADAKNMIGKTPGKIVAVRPMKDGVIADYDMTTDLLKHIMKKAAKSIGMSFRKPNVVVCTPSGSTAVERRAISDAVKNCGAKNVHLIEEPVAAAIGADLPVDEPVANVVVDIGGGTTEVAIISFGGVVSCHSIRIGGDQLDEDIVSFVRKKYNLLIGERTAEQVKMEIGHALIEHIPEAM--EIRGRDLVTGLPKTIMLQSNEIQDAMRESLLHILEAIRATLEDCPPELSGDIVDRGVILTGGGALLNGIKEWLTEEIVVPVHVAQNPLESVAIGTGRSLEVIDKLQKAIK 4CZL Chain:A ((3-326)) ---SNDIAIDLGTANTLIYQKGKGIVLNEPSVVALRNVGGRKVVHAVGIEAKQML------MEAIRPMRDGVIADFEVAEEMIKYFIRKVHNRKGSG--NPKVIVCVPSGATAVERRAINDSCLNAGARRVGLIDEPMAAAIGAGLPIHEPTGSMVVDIGGGTTEVAVLSLSGIVYSRSVRVGGDKMDEAIISYMRRHHNLLIGETTAERIKKEIGTARAPADGEGLSIDVKGRDLMQGVPREVRISEKQAADALAEPVGQIVEAVKVALEATPPELADDIADKGIMLTGGGALLRGLDAEIRDHTGLPVTVADDPLSCVALGCGKVLE----------

General information: TITO was launched using: RESULT: Template: 4CZL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1841 -182301 -99.02 -580.57 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -99.02 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.573

(partial model without unconserved sides chains): PDB file : Tito_4CZL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CZL-query.scw PDB file : Tito_Scwrl_4CZL.pdb: