Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFYTIKLPKFLGGIVRAMLGSFRKD 4PF3 Chain:A ((254-271)) --IISDQLPKVESGNVKPLY------

General information: TITO was launched using: RESULT: Template: 4PF3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 7 -848 -121.07 -47.08 target 2D structure prediction score : 0.28 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -121.07 2D Compatibility (Sec. Struct. Predict.) : 0.28 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.354

(partial model without unconserved sides chains): PDB file : Tito_4PF3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PF3-query.scw PDB file : Tito_Scwrl_4PF3.pdb: