Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTIHITHPHHLIKEEQAKSVMALGYFDGVHLGHQKVIGTAKQIAEEKGLTLAVMTFHPHPSHVLGRDKEPKDLITPLEDKINQIEQLGTEVLYVVKFNEVFASLSPKQFIDQYIIGLNVQHAVAGFDFTYGKYGKGTMKTMPDDLDGKAGCTM--VEKLTEQDKKISSSYIRTALQNGDVELANVLLGQPYFIKGIVIHGDKRGRTIGFPTANVGLNNSYIVP-PTGVYAVKAEV-NGEVYNGVCNIGYKPTFYEKRPEQPSIEVNLFDFNQEVYGAAIKIEWYKRIRSERKFNGIKELTEQIEKDKQEAIRYFSNLRK 2I1L Chain:A ((2-277)) --------------------VVSIGVFDGVHIGHQKVLRTMKEIAFFRKDDSLIYTISYPPEYFL---PDFPGLLMTVESRVEMLSRYARTV--VLDFFRI-KDLTPEGFVERYLSG--VSAVVVGRDFRFGKNASGNASFLR-----KKGVEVYEIEDVVVQGKRVSSSLIRNLVQEGRVEEIPAYLGRYFEIEGIV-----------FPTANIDRGNEKLVDLKRGVYLVRVHLPDGKKKFGVMNVGFR---------NVKYEVYILDFEGDLYGQRLKLEVLKFMRDEKK----EELKAAIDQDVKSA---------

General information: TITO was launched using: RESULT: Template: 2I1L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1181 -159997 -135.48 -639.99 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -135.48 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_2I1L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I1L-query.scw PDB file : Tito_Scwrl_2I1L.pdb: