Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDMNEHRNRRLKLIMISAT----FGG-LLFGYDTGVINGALPFMAR-------------PDQLDLTPVTEGLVTSILLLGAAFGALLCGRLADRYGRRKMILNLSFLFFLASL---GTALAPNVFIMAVFRFLLGLAVGGASAMVPAFLAEMAPHEKRGRMVTQNELMIVGGQFLAYVFNAILGVTMANTGHVWRYMLVICAVPAIMLFASMLKVPESPRWLISKGKNSEALR-VLKQIREDKRAEAECREIQEAVEKDTALEKAS-LKDFSTPWLRRLLWIGIGVAIV---NQITGVNSIMYYGTQILKESGFGTKAALIANIGNGLISVIAVIFGIWLVGKVRRRPILLIGLAGTTTALLLIAIFSIVLDGSMAL-------PYVVLSLTVLFLAFMQGCVGPVTWLVIAEIFPQRLRGLGSGISVFFLWILNFVIGFAFPILLSSVGLSFTFFIFVALGVLAIGFVYKFMPETKGRTLEELEEHFRSRHDHNTPEQSVIEV 4YB9 Chain:D ((19-462)) ----------------LATLIAAFGSSFQYGYNVAAINSPSEFMKDFYAYTYYDRVGEYMNEFYLT-LLWSVTVSMFPFGGFLGSLMVGPLVNNLGRKGTLLFNNIFSIVPALLMGFSELAKSFEMIIVARVLVGICAGLSSNVVPMYLGELAPKNWRGALGVVPQLFITIGILVAQIFG--LRSLLANE-EGWPILLGLTGIPAVLQLLFLPFFPESPRYLLIQKKDEAAAKSALRRL-------AEIEEILEEDRAEKAVGFISVLKLFK---MRSLRWQVISIIVLMAGQQLSGVNAIYYYADQIYLSAGVNEDDVQYVTAGTGAVNVLITVCAIFVVELMGRRFLLLLGFSVCFTA-------CCVLTGALALQDVISWMPYVSIACVISYVIGHALGPSPIPALLVTEIFLQSSRPAAYMVAGTVHWLSNFTVGLVFPFIQVGLG-AYSFVIFAVICLLTTVYIFLIIPE-----------------------------

General information: TITO was launched using: RESULT: Template: 4YB9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1947 -213778 -109.80 -529.15 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain D : 0.74 3D Compatibility (PKB) : -109.80 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.372

(partial model without unconserved sides chains): PDB file : Tito_4YB9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YB9-query.scw PDB file : Tito_Scwrl_4YB9.pdb: