Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFLL-----DLFTNWTFDKVLDYTLAAVIWSVFKSRSKQNKYPGYFEKIRRYRNLHPLLRSLSMRVLLSITIHPYMFS 2W8X Chain:A ((1-72)) FNCNKREGPCSQRSLCECDPNLQLGRHSDQLWHYNLRTNRCERGGY----RDNCNSHS---SSGACVMACERIHHHHHH

General information: TITO was launched using: RESULT: Template: 2W8X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 298 8516 28.58 127.10 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 28.58 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.053

(partial model without unconserved sides chains): PDB file : Tito_2W8X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W8X-query.scw PDB file : Tito_Scwrl_2W8X.pdb: