Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPTEIYVDGASAGNPGPSGIGIFIKHEGKAESFSIPIGVHTNQEAEFLALIEGMKLCATRGYQSVSFRTDSDIVERATELEMVKNITFQPF----------VEEIIRLKAAFPLFFIK--WIPGK----QNQKADLLAKEAIRLNEKN 2Z1G Chain:A ((6-140)) ---EIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYERTTNNRMELMAAI--VALEALKEHCEVILSTDSHYVRKGI-TEWIHNWKKRGWKKADKKPVKNVDLWKRLDAALGQHQIKWEWVKGHAGHPENERCDELARAA-------

General information: TITO was launched using: RESULT: Template: 2Z1G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 504 -24698 -49.00 -207.54 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -49.00 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_2Z1G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z1G-query.scw PDB file : Tito_Scwrl_2Z1G.pdb: