Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHRGKGMKFVGDSRIPAEKKPNIPKDYSEYPGKTEAFWPNFLLKEWMVGAVFLIGFLV---LTIVHQPPLERMADPTDTGYIPLPDWYFLFLYQLLKYEYAAGSFTVVGAMIMPGLAFGALLLAPFLDRGTE-RRPWKRPVAVGMMLLAISAAVFLTWQSVATHDWAKAEEQGKITKEADIDTNAEGYKVFKEQGCISCHGDNLQGGAAGPSLVDSGLKPDEIKKIAVEGKGKMPAGVFKGNDKQLEELAKFISETTAK 1Q90 Chain:D ((29-143)) ----------------------------------EPAWPNDLL--YMFPVVILGTFACVIGLSVLDPAAMGEPANPFATPLEILPEWYFYPVFQILR----VVPNKLLGVLLMAAVPAG-LITVPFIESINKFQNPYRRPIATILFLLGTLVAVWL-------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1Q90.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 120 -22083 -184.02 -198.94 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain D : 0.69 3D Compatibility (PKB) : -184.02 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.166

(partial model without unconserved sides chains): PDB file : Tito_1Q90.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Q90-query.scw PDB file : Tito_Scwrl_1Q90.pdb: