Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMIKQCVICLSLLVFGTTAAHAEETPLVTARHMSKWEEIAVKEAKKRYPLAQVLFKQKVWDRKRKDEAVKQYHLTLREGSKEFGVFVTISFDPYSQKVNKIAILEEYQ 4FR9 Chain:A ((37-91)) --------------------------------------AIQDAL--LYPNATAI-WEQ----------GVYYVADCQADGR----E-EVWFDA-NANWLMTETE----

General information: TITO was launched using: RESULT: Template: 4FR9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 160 -19446 -121.54 -413.74 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -121.54 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.585

(partial model without unconserved sides chains): PDB file : Tito_4FR9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FR9-query.scw PDB file : Tito_Scwrl_4FR9.pdb: