Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQQLTFYSYPSCTSCRKTKHWLKAHQIEFNERHLFRETPTREELKYILSLTTEGIDEILATRSQTFKNLNLNIEEMTVNEVLELLIEKPKLLRRPILVDNKKLVIGYNPGELLKLSKKKTVHQSA 1S3D Chain:A ((2-96)) ----ITIYHNPACGTSRNTLEMIRNSGTEPTIILYLENPPSRDELVKLIADMGISVRALLAKNVEPYEQLGLAEDKFTDDQLIDFMLQHPILINRPIVV---------------------------

General information: TITO was launched using: RESULT: Template: 1S3D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 355 -67465 -190.04 -710.15 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -190.04 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.586

(partial model without unconserved sides chains): PDB file : Tito_1S3D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1S3D-query.scw PDB file : Tito_Scwrl_1S3D.pdb: