Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTIELNKRQEHILQIVKENGPITGEHIAEKLNLTRATLRPDLAILTMSGFLEARPRVGYFYTGKTGTQLLADKLKKLQVKDFQSIPVVIHENVSVYDAICTMFLEDVGTLFVVDRDAVLVGVLSRKDLLRASIGQQELTSVPVHIIMTRMPNITVCRREDYVMDIAKHLIEKQIDALPVIKDTDKGFEVIGRVTKTNMTKILVSLSENEIL 3LFZ Chain:A ((95-205)) ---------------------------------------------------------------------------------------ITLKENADIDEAIETFLTKNVGGAPIVNDENQLISLITERDVIRALLDKIDENEV-IDDYITR--DVIVATPGERLKDVARTMVRNGFRRLPVVSEG----RLVGIITSTDFIKLLGS-------

General information: TITO was launched using: RESULT: Template: 3LFZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 263 -49154 -186.90 -442.82 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -186.90 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_3LFZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LFZ-query.scw PDB file : Tito_Scwrl_3LFZ.pdb: