Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGLIGLGKMGINIGKQFIDRNHQVVGYDVNQAAVDELKAYGAEGTT----NLKEFISLLHPPRILWVMVPHGI-VDAVLRDVSPLLSKGDMIIEAGNSHYKESIRRYNQMKEAGIHYLDAGTSGGMEGARHGACFMVGGDHEAWEIVEPLFRDTAV-----ENGYLYAGEAGSGHFLKMIHNGIEYGMMAAIGEGFEVLENSQFDFDYEKVARV---WNHGSVIRSWLMGLTERAFAKDAK-----LDQIKGIMHSSGEGKWTVETALDLQTATPVIAMSLMMRYRSLTDDTFTGKVVAALRNEFGGHATEKK 2ZYG Chain:A ((5-294)) -QIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVIAENPGKKLVPYYTVQEFVESLETPRRILLMVKAGAGTDSAIDSLKPYLDKGDIIIDGGNTFFQDTIRRNRELSAEGFNFIGTGVSGGEEGTLKGPSIMPGGQKEAYELVAPILKQIAAVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYALLKGG-LALSNEELAQTFTEWNEGE-LSSYLIDITKDIFTKKDEEGKYLVDVILDEAANKGTGKWTSQSSLDLGEPLSLITESVFARYISSLKD----------------------

General information: TITO was launched using: RESULT: Template: 2ZYG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1297 8389 6.47 30.84 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 6.47 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_2ZYG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZYG-query.scw PDB file : Tito_Scwrl_2ZYG.pdb: