Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKGQLFIGALTCLVASMSWGAMFPVADHALEFVDPFYFSFIRYGVVTIMLVILLLVREGKKSFHLEGKAKWIILFGVMAFTIYNVLIFLGQRLMGKSGIMTASIAEALMPMLSIVILWGYKHVKPKKYTMISILIAFLGASMVITKGNISFFFSLGDHLFSILFIFIGVLGWVVYTMGGQIFREWSTLRYSTLTCLFGTAITGIMTAILTAQGYVSVPSIKVIAAIKYDFLFMITLPGIIALLSWNYGIKILSSINGILFINFVPITTLLIMVIKGYNITAFDIVGTLFVIIGLILNNIYQRKEDYKQVLQKEKTNLTIT 2MKV Chain:A ((30-50)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IFAGLAFIVGLLILLSRRFRS----------------

General information: TITO was launched using: RESULT: Template: 2MKV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1 95 94.50 4.50 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain A : 0.36 3D Compatibility (PKB) : 94.50 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.36 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_2MKV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MKV-query.scw PDB file : Tito_Scwrl_2MKV.pdb: