Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIFSLALISLFAVILLAACGSKGSNGEASKESKKDTLAAIKDNDKIVFGVKTDTRLFGLKNPSSGEIEGFDIDIAKQIAKDILGDEKKAQFKEVTSKTRIPMLQNGDIDAIVATMTITEERKKEVDFSDVYFEAGQSLLVKKGSKIKSVENLGKGSKVLAVKGSTSSQNIREKAPEASVLEFENYAEAFTALKSGQGDALTTDNAILYGMADENKNYQLTGKPFTD-EPYGIAVKKGQSALAKEINASLKKMKSDGRYDEIYKKWIKEDPAE 1XT8 Chain:A ((34-265)) -----------------------------------LNSLDKIKQNGVVRIGVFGDKPPFGYVD-EKGNNQGYDIALAKRIAKELFGDENKVQFVLVEAANRVEFLKSNKVDIILANFTQTPQRAEQVDFCSPYMKVALGVAVPKDSNITSVEDL-KDKTLLLNKGTTADAYFTQNYPNIKTLKYDQNTETFAALMDKRGDALSHDNTLLFAWVKDHPDFKMGIKELGNKDVIAPAVKKGDKELKEFIDNLIIKLGQEQFFHKAYDETLK-----

General information: TITO was launched using: RESULT: Template: 1XT8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1279 -62302 -48.71 -269.70 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -48.71 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_1XT8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1XT8-query.scw PDB file : Tito_Scwrl_1XT8.pdb: