Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYLDYAATTPICEEALTVYQKLSMDMYGNASSLHDAGGKAKHILEYCREKIANIIGGEASGIYFTSGGTESNFLAIQSLL---------NGLPKTKRHFITTAMEHQSIH-NCAAFLEQQGYDVTVIEPNEYGLITE-EILLTHIRPETGLVSIQHANSETGIIQPIQHLSSYLHN----------KGILLHCDAVQTFGKIPINTKNLGVDALSMSSHKIHGPKGVGAVYIRPDVPWKPVYPLT---THEYGFRAGTVNVPGIGAFTAAAELIVSEMEKQISRNEALRTYFLDQIRIR--SLPVTLAADTSKAECLPHIIGCFFH----SFEGQYVMLECNRSNICISTGSACSAGYHG--PSETMKALRKTEQEALQFIRISFGRHTTAEQLEQLLHTFTVLWEQKKGEFDIDRRIKANGRQQA 3GZD Chain:C ((27-429)) -VYMDYNATTPLEPEVIQAMTKAMWEAWGNPSSPYSAGRKAKDIINAARESLAKMIGGKPQDIIFTSGGTESNNLVIHSVVKHFHANQTS----GAKPHFITSSVEHDSIRLPLEHLVEEQVAAVTFVPVSKVSGQTEVDDILAAVRPTTRLVTIMLANNETGIVMPVPEISQRIKALNQERVAAGLPPILVHTDAAQALGKQRVDVEDLGVDFLTIVGHKFYGPR-IGALYIRGLGEFTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAAELVTQNCEAYEAHMRDVRDYLEERLEAEFGQKRIHLNSQFPGTQRLPNT--CNFSIRGPRLQGHVVLAQCRV--LMASVGAAC------DQPSPVLLSYGVPFDVARNALRLSVGRSTTRAEVDLVVQDL-------------------------

General information: TITO was launched using: RESULT: Template: 3GZD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1951 -22624 -11.60 -63.91 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : -11.60 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_3GZD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GZD-query.scw PDB file : Tito_Scwrl_3GZD.pdb: