Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYLDNSSTTKPYDEVLNVYEQTSSRYFGNPSSLHRYGAETEQLLQAAKNQIKRSLGLKKYDIVFTSGATEANN-------------AALKGAALSKIK--TGKHIIATSIEHPSVTESLEQLKELFGFDITYLSVNE-DGFVSIEDLKAAIRPDTVLVSMMHVNNEVGSVQPIEAAGEVLKEHSNI---------LFHVDYVQGIYKVPLAIEKAGIDLCSISGHKFHGLKGTGALIVK---EGTRLIPLITGGSQQKGIRAGTEHTAGAVSLAKAINLASADFDTRLDTMTAVKELFMNRLSEIEGVVINT----PQMNSAPHIINFSVPGIKAEVLLHMLEEQDIFVSTTSACSAKEHKPSKVLLEMGKGEQIAGSSIRISLNYSQTSDVAEPFMNALRPGIKKLREMMR 3A9X Chain:A ((20-428)) -VYMDYNATTPLEPEVIQAVTEAMKEAWGNPSSSYVAGRKAKDIINTARASLAKMIGGKPQDIIFTSGGTESNNLVIHSTVRCFHEQQTLQG-------EGTRPHFITCTVEHDSIRLPLEHLVEDQVAEVTFVPVSKVNGQVEVEDILAAVRPTTCLVTIMLANNETGVIMPISEISRRIKALNQIRAASGLPRVLVHTDAAQALGKRRVDVEDLGVDFLTIVGHKFYGPR-IGALYVRGVGKLTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAADLVSENCETYEAHMRDIRDYLEERLEAEFGKRIHLNSRFPGVERLPNTCNFSIQGSQLRGYMVLAQCQTLLASVGA------------------PVDVARNAVRLSVGRSTTRAEVDLIVQDLKQAVNQL-----

General information: TITO was launched using: RESULT: Template: 3A9X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1862 17550 9.43 50.29 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 9.43 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.498

(partial model without unconserved sides chains): PDB file : Tito_3A9X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A9X-query.scw PDB file : Tito_Scwrl_3A9X.pdb: