Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGRTLFFRRLFDYYKYQFKVLHAVIDWTVALYIVLPAIAFVIYQYIDLMNGRGLLYEWSEVAEWRWLYAVCVLIMFTGSIRTFLMEADKVFLLQKKEIIYQLKRYALLYSFLATLAKWLLLFFIVLPLISHSVLITFAESTALLCYLFGLHIFFLSLKQDRIRKPRSISRWIGDTLVRAILFAGSAILIVFTERHLLALFGILFLFFSVIRSLKKTASFTAFEAEVTEEKKSRLALAGLVMMMSQEAGMPKVKDRMRRKPLLYRNSKRIFKRRTICTGYKELFFKVMLRNGEYARQMYMLLSAFTVLIFVSPIWLKVIALLVYTGVCRYILTLIFDKVMDAPFLIGTDKESDEYYRARKSCINILHYAFAACCFLAAAVSLLFT 1ZRV Chain:A ((5-20)) -------------------------------------------------------------------------------------KVADKVLLLKQLRIMR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1ZRV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 11 -1029 -93.50 -64.28 target 2D structure prediction score : 0.19 Monomeric hydrophicity matching model chain A : 0.39 3D Compatibility (PKB) : -93.50 2D Compatibility (Sec. Struct. Predict.) : 0.19 1D Compatibility (Hydrophobicity) : 0.39 QMean score : 0.649

(partial model without unconserved sides chains): PDB file : Tito_1ZRV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZRV-query.scw PDB file : Tito_Scwrl_1ZRV.pdb: