Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMQEETFYSVRMRASMNGSHEDGGKHISGGERLIPFHEMKHTVNALLEKGLSHSRGKPDFMQIQFEEVHESIKTIQPLPVHTNEVSCPEEGQKLARLLLEKEGVSRDVIEKAYEQIPEWSDVRGAVLFDIHTGKRMDQTKEKGVRVSRMDWPDANFEKWALHSHVPAHSRIKEALALASKVSRHPAVVAELCWSDDP---DYITGYVAGKKMGYQRITAMKEYGTEEGCRVFFIDGSND----VNTYIHDLEKQPILIEWEEDHDS 3ZFI Chain:A ((44-102)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SDFFRFYRSADKQEKEKARIYLLGVLDATE-------------GKSWCQYSQLQTVTLQEFVFEFFNKLPAA---------

General information: TITO was launched using: RESULT: Template: 3ZFI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 89 -8703 -97.78 -167.36 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -97.78 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.313

(partial model without unconserved sides chains): PDB file : Tito_3ZFI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZFI-query.scw PDB file : Tito_Scwrl_3ZFI.pdb: