Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNTPTQLEPNVPVTRSHSMGFVILISC--AAGLGGLLYGYDTAVISGAIGFLKDLYSLSPFME--------------GLVISSIMIGGVVGVGISGFLSDRFGRRKILMTAALLFAISAIV---SALSQDVSTLIIARIIGGLGIGMGSSLSVTYITEAAPPAIRGSLSSLYQLFTILGISATYFINLAVQRSGTYEWGVHTGWRWMLAYGMVPSVIFFLVLLVVPESPRWLAKAGKTNEALK-ILTRINGETVAKEELKNIENSLKIEQ-MGSLS--QLFK-PGLRKALVIGILLALFNQVIGMNAITYYGPEIFKMMGFGQNAGFVTTCIVGVVEVIFTVIAVLLIDKVGRKKLMSIG-SAFMAIFMILIGTSFYFELTSGI--MMIVLILGFVAAFCVSVGPITWIMISEIFPNHLRARAAGIATIFLWGANWAIGQFVPMMIDSFGLAYTFWIFAVINILCFLFVVTICPETKNKSLEEIEKLWIK 4YB9 Chain:D ((19-462)) -------------------------LATLIAAFGSSFQYGYNVAAINSPSEFMKDFYAYTYYDRVGEYMNEFYLTLLWSVTVSMFPFGGFLGSLMVGPLVNNLGRKGTLLFNNIFSIVPALLMGFSELAKSFEMIIVARVLVGICAGLSSNVVPMYLGELAPKNWRGALGVVPQLFITIGILVAQIFGLRSLLAN------EEGWPILLGLTGIPAVLQLLFLPFFPESPRYLLIQKKDEAAAKSALRRL-------AEIEEILEEDRAEKAVGFISVLKLFKMRSLRWQVISIIVLMAGQQLSGVNAIYYYADQIYLSAGVNEDDVQYVTAGTGAVNVLITVCAIFVVELMGRRFLLLLGFSVCFTACCVLTGALALQDVISWMPYVSIACVISYVIGHALGPSPIPALLVTEIFLQSSRPAAYMVAGTVHWLSNFTVGLVFPFIQVGLG-AYSFVIFAVICLLTTVYIFLIIPE---------------

General information: TITO was launched using: RESULT: Template: 4YB9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1997 -189172 -94.73 -461.39 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain D : 0.75 3D Compatibility (PKB) : -94.73 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_4YB9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YB9-query.scw PDB file : Tito_Scwrl_4YB9.pdb: