Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLKVMTVFGTRPEAIKMAPLVLELKKYPEIDSYVTVTAQHRQMLDQVL--DAFHIKPDFDLNIMKERQTLAEITSNALVRLDELFKDIKPDIVLVHGDTTTTFAGSLAAFYHQIAVGHVEAGLRTGNKYSPFPEELNRQMTGAIADLHFAPTGQAKDNLLKENKKADSIFVTGNTAIDALNTTVRDGYSHPVLDQVG--EDKMILLTAHRRENLGEPMENMFKAIRRIVGEFEDVQVVYPVHLNPVVREAAHKHFGD-SDRVHLIEPLEVIDFHNFAAKSHFILTDSGGVQEEAPSLGKPVLVLRDTTERPEGVEAGTLKLAGTDEENIYQLAKQLLTDPDEYKKMSQASNPYGD-GEASRRIVEELLFHYGYRKEQPDSFTGK 4HWG Chain:A ((10-376)) ---LKVMTIVGTRPELIKLCCVISEFDKHTK-HILVHTGQNYAYELNQVFFD-MGIRKPDYFLEVAADNT--AKSIGLVIEKVDEVLEKEKPDAVLFYGDTNSCLS-AIAAKRRKIPIFHMEAGNRCFDQR--VPEEINRKIIDHISDVNITLTEHARRYLIAEGLPAELTFKSGSHMPEVLDRFMPKILKSDILDKLSLTPKQYFLISSHRE---KNNLKELLNSLQMLIKEY-NFLIIFSTHPRTKKRLEDLEGFKELGDKIRFLPAFSFTDYVKLQMNAFCILSDSGTITEEASILNLPALNIREAHERPEGMDAGTLIMSGFKAERVLQAVKTITEEHDNNKRTQGLVPDYNEAGLVSKKILRIVLSYVDY-----------

General information: TITO was launched using: RESULT: Template: 4HWG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1958 34370 17.55 96.82 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 17.55 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.436

(partial model without unconserved sides chains): PDB file : Tito_4HWG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HWG-query.scw PDB file : Tito_Scwrl_4HWG.pdb: