Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKAVSVILTLSLFLLTACSLEPPQWAKPSNSGNKKEFTIGLSVSTLNNPFFVSLKKGIEKEAKKRGMKVIIVDAQNDSSKQTSDVEDLIQQGVDALLINPTDSSAISTAVESANAVGVPVVTIDRSAE-QGKVETLVASDNVKG----GEMAAAFIADKL--GKGAKVAELEGVPGASATRERGSGFHNIADQ--KLQVVTKQSADFDRTKGLTVMENLLQGHPDIQAVFAHNDEMALGALEAINSSGK-DILVIGFDGNKDALASIKD-RKLSATVAQQPELIGKLATEAADDILHGKKVQKTISAPLKLETQK 2FN8 Chain:A ((5-266)) ---------------------------------------MAIVISTLNNPWFVVLAETAKQRAEQLGYEATIFDSQNDTAKESAHFDAIIAAGYDAIIFNPTDADGSIANVKRAKEAGIPVFCVDRGINARGLAVAQIYSDNYYGGVLMGEYFVKFLKEKYPDAKEIPYAELLGILSAQPTWDRSNGFHSVVDQYPEFKMVAQQSAEFDRDTAYKVTEQILQAHPEIKAIWCGNDAMALGAMKACEAAGRTDIYIFGFDGAEDVINAIKEGKQIVATIMQFPKLMARLAVEWADQYLRGER---------------

General information: TITO was launched using: RESULT: Template: 2FN8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1474 -15672 -10.63 -62.44 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -10.63 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_2FN8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FN8-query.scw PDB file : Tito_Scwrl_2FN8.pdb: