Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEIKLKYDTVIKTLDSVKDALADVSIGA---AGSNGKNSL------DYTKKYHEREENIKTMLGDYKKAVQKNIEDTKDNVDSLKEQDEAIAVK 2VS0 Chain:A ((3-85)) --MIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQ--GEIAANWEGQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQE--------

General information: TITO was launched using: RESULT: Template: 2VS0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 123 12524 101.82 169.24 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 101.82 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.436

(partial model without unconserved sides chains): PDB file : Tito_2VS0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VS0-query.scw PDB file : Tito_Scwrl_2VS0.pdb: