Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKGLGKGINALFNQVDLSEETVEEIKIADLRPNPYQPRKHFDDEALAELKESVLQHGILQPLIVRKSLKGYDIVAGERRFRAAKLAGLDTVPAIVRELSEALMREIALLENLQREDLSPLEEAQAYDSLLKHLDLTQEQLAKRLGKSRPHIANHLRLLTLPENIQQLIAEGTLSMGHGRTLLGLKNKNKLEPLVQKVIAEQLNVRQLEQLIQQLNQNVPRETKKKEPVKDAVLKERESYLQNYFGTTVNIKRQKKKGKIEIEFFSNEDLDRILELLSERES 1VZ0 Chain:F ((23-211)) ----------------------VVRLPLASIRPNPRQPRKRFAEESLKELADSIREKGLLQPLLVRPQGDGYELVAGERRYRAALMAGLQEVPAVVKDLTDREALELALVENLQREDLSPVEEARGYQALL-EMGLTQEEVARRVGKARSTVANALRLLQLPPEALEALERGEITAGHARALLMLEPEDRLWGL-KEILEKGLSVRQAEALRE---------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1VZ0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 614 -77156 -125.66 -408.23 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain F : 0.79 3D Compatibility (PKB) : -125.66 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.716

(partial model without unconserved sides chains): PDB file : Tito_1VZ0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VZ0-query.scw PDB file : Tito_Scwrl_1VZ0.pdb: