Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEGFAGKVAVVTGAGSGIGQALAIELARSGAKVAISDVDTDGLADTEHRLKAISTP-VKTDRLDVTEREAFLAYADAVNEHFGTVNQIYNNAGI---AFTGDIEVSQFKDIERVMDVDFWGVVNGTKAFLPHLIASGDGHVINISSVFGLFSAPGQAAYNSAKFAVRGFTEALRQEMALAGHPVKVTTVHPGGVKTAIARNATAAEGLDQAELAETFDKRVAHLSPQRAAQIILTGVAKNKARVLVGVDAKVLDLVVRLTGSGYQRIFPIITGRLIPRPR 4NBT Chain:A ((2-217)) -KKLEGKVAVITGGAKGLGQAIALAYAEEGAKVIAGD-----LGDLTY-----SHPNVEGMYLNVTDVTGVEKFYQSVIDKYGKIDILVNNAGITKDAMTRKMTEAQW---DAVIDVNLKGVFNLTRLVGPQMQTNGYGSIINISSVVGVFGNIGQANYAATKAGVIGLTMTWAKEFALKGANVRVNAIAPGYIMTDILK--TVPQDLLDKFAALTMLNRLGQ--PEEIAKVAL----------------------------------------------

General information: TITO was launched using: RESULT: Template: 4NBT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -72319 for 1735 contacts (-41.7/contact) + 2D Compatibility (PS) -23735 + (NN) -12456 + (LL) 4444 1D Compatibility (HY) -18400 + (ID) 4000 Total energy: -126466.0 ( -72.89 by residue) QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_4NBT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NBT-query.scw PDB file : Tito_Scwrl_4NBT.pdb: