Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIAISGGGTGGHIYPALAFIKEVQRRHPNVEFLYIGTENGLEKKIVERENIPFRSIEITGFKRKL---------------SF--ENVKTVMR-------FLKGVKKSKSYLAEFKPDAVIGTGGYVCGPVVYAAAKMGIPTIVHEQNSLP-GI----TNKFLSKYVNKV----------------AICFEEAKSHFPS---EKVVFTGNPRASEVVSIKTGRSLAEFG-LSEDKKTVLIFGGSRGAAPINRAVIDMQDVLKTRDYQVLYITGEVHYEKVMNELKSKGAADNMVTKPFLHQMPEYLKAIDVIVARAGATTIAEITALGIPSVLIPSPYVTANHQEVNARSLGQHDAAIVLKETELSGEKLIEALDRIVLNEQTLKEMSERTKSLGVPDAAARLYSVLEELKK 3OTG Chain:A ((21-406)) MRVLFASLGTHGHTYPLLPLATAAR-AA-GHEVTFATGEGF-AGTLRKL-GFEPV--ATGMPVFDGFLAALRIRFDTDSPEGLTPEQLSELPQIVFGRVIPQRVFDELQPVIERLRPDLVVQEISN--YGAGLAALKAGIPTICHGVGRDTPDDLTRSIEEEVRGLAQRLGLDLPPGRIDGFGNPFIDIFPPSLQEPEFRARPRRHELRPVPFAEQGDLP----A-WLSSRDTARPLVYLTLGTSSGGTV-EVLRAAIDGLAGLDADVLVASGPSLDVSGL-----GEVPANVRLESWV-PQAALLPHVDLVVHHGGSGTTLGALGAGVPQLSFPWA----GDSFANAQAVAQAGAGDHLLPDNISPDSVSGAAKRLLAEESYRAGARAVAAEIAAMPGPDEVVRLLPGF--

General information: TITO was launched using: RESULT: Template: 3OTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1932 -199154 -103.08 -590.96 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -103.08 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_3OTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OTG-query.scw PDB file : Tito_Scwrl_3OTG.pdb: