Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNQTARSSKKERYANLIPMEELHSEKDRLFPFPIYDKLRRESPVRYDPLRDCWDVFKYDDVQFVLKNPK------------------------LFSSKRGIQTESILTMDPPKHTKLRALVSRAFTPKAVKQLETRIKDVTAFLLQEARQKSTIDIIEDFAGPLPVIIIAEMLGAPI-EDRHLIKTYSDVLVAGAKDSSDKAVADMVHNRRDGHAFLSDYFRDILSKRRAEP-KEDLMTMLLQAEIDGEYLTEEQLIGFCILLLVAGNETTTNLIANAVRYLTEDSVVQQQVRQNTDN--VANVIEETLRYYSPVQ-AIGRVATEDTEL-GGVFIKKGSSVISWIASANRDEDKFCKPDCFKIDR-PSYPHLSFGFGIHFCLGAPLARLEANIALSSLLSMSACIEKAAHDEKLEAIPSPFVFGVKRLPVRITFK- 1IO7 Chain:A ((1-366)) -----------------------------M--YDWFSEMRKKDPVYYDG--NIWQVFSYRYTKEVLN--NFSKFSSDLTGYHERLEDLRNGKIR----FDIPTRYTMLTSDPPLHDELRSMSADIFSPQKLQTLETFIRETTRSLLDSIDP-REDDIVKKLAVPLPIIVISKILGLP-IEDKEKFKEWSDLVAFRLGK------PGEIFELGKKYLELIGYVKDHLN-----SG-TEVVSRVVNS-----NLSDIEKLGYIILLLIAGNETTTNLISNSVIDFTRFN-LWQRIREE---NLYLKAIEEALRYSPPVMRT-VRKTKERVKLG-DQTIEEGEYVRVWIASANRDEEVFHDGEKFIPDRN-PNPHLSFGSGIHLCLGAPLARLEARIAIEEFSKRFRHIEILD----TEKVPNEVLNGYKRLVVRLK--S

General information: TITO was launched using: RESULT: Template: 1IO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1617 -192227 -118.88 -575.53 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -118.88 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_1IO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IO7-query.scw PDB file : Tito_Scwrl_1IO7.pdb: