Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVHIGAEKGQIADTVLLPGDPLRAKFIAETYLENVECYNEVRGMYGFTGTYKGKKISVQGTGMGVPSISIYVNELIQSYDVQNLIRVGSCGAIRKDVKVRDVILAMTSSTDSQMNRVAFGSVDFAPCADFELLKNAYDAAKDKGVPVTVGSVFTADQFYND-DSQIEKLAKYGVLGVEMETTALYTLAAKHGRKALSILTVSDHVLT-GEETTAEERQTTFHDMIEVALHSVSQ 3UAW Chain:A ((2-233)) -SVHIEAKQGEIAESILLPGDPLRAKYIAETFLEDVTCYNNVRGMLGFTGTYKGKRVSVQGTGMGVPSISIYVNELIQSYGVKNLIRVGTCGAIQKDVKVRDVIIAMTACTDSNMNRLTFPGFDFAPAANFDLLKKAYDAGTEKGLHVRVGNVLTADVFYRESMDMVKKLGDYGVLAVEMETTALYTLAAKYGVNALSVLTVSDHIFTGEETTSEER-QTTFNEMIEIALDAAI-

General information: TITO was launched using: RESULT: Template: 3UAW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1425 -121982 -85.60 -530.35 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.94 3D Compatibility (PKB) : -85.60 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.572

(partial model without unconserved sides chains): PDB file : Tito_3UAW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UAW-query.scw PDB file : Tito_Scwrl_3UAW.pdb: