Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLEEKTIAKEQIFSGKVIDLYVEDVELPNGKASKREIVKHPGAVAVLAVTDEG-KIIMVKQFRKPLERTIVEIPAGKLE-KGEEPEYTALRELEEETGYTAKKLTKITAFYTSPGFADEIVHVFLAEELSVLEEKRELDEDEFVEVMEVTLEDALKLVESREVYDAKTAYAIQYLQLKEALQAQK 1MK1 Chain:A ((6-184)) -----FETISSETLHTGAIFALRRDQVR-----IVTREVVEHFGAVAIVAMD-DNGNIPMVYQYRHTYGRRLWELPAGLLDVAGEPPHLTAARELREEVGLQASTWQVLVDLDTAPGFSDESVRVYLATGLREVGR----------TMGWYPIAEAARRVLRGEIVNSIAIAGVLAVHAVTTGFA--

General information: TITO was launched using: RESULT: Template: 1MK1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 762 -21819 -28.63 -134.68 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -28.63 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_1MK1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MK1-query.scw PDB file : Tito_Scwrl_1MK1.pdb: