Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRVLLIDDHEMVRMGLAAFLEAQPDIEVIGEASDGSEGVRLAVELSPDVILMDLVMEGMDGIEATKQICRELSDPKIIVLTSFIDDDKVYPVIEAGALSYLLKTSKAAEIADAIRAASKGEPKLESKVAGKVLSRLRHSGENALPHESLTKRELEILCLIAEGKTNKEIGEELFITIKTVKTHITNILSKLDVSDRTQAAVYAHRNHLVN 1YIO Chain:A ((5-200)) -PTVFVVDDDMSVREGLRNLLRSA-GFEV-ETFDCASTFLEHRRPEQHGCLVLDMRMPGMSGIELQEQLTAISDGIPIVFITAHGDIPMTVRAMKAGAIEFLPKPFEEQALLDAIEQGLQLNAERRQA--RETQDQ------LEQLFSSLTGREQQVLQLTIRGLMNKQIAGELGIAEVTVKVHRHNIMQKLNVRSLANLVHLVEKY----

General information: TITO was launched using: RESULT: Template: 1YIO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 807 -105877 -131.20 -540.19 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -131.20 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_1YIO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YIO-query.scw PDB file : Tito_Scwrl_1YIO.pdb: