Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIAISGGGTGGHIYPALAFIKEVQRRHPNVEFLYIGTENGLEKKIVERENIPFRSIEITGFK-RKL-------SF-ENVK----TVMR-FLKGVKKSKSYLAEFKPDAVIGTGGYVCGPVVYAAAKMGIPTIVHEQNSLPGIT-----NKFLSKYV-----------NKVAICFEEA-KSHFPS--EKVVFTGNPRASEVVSIKTGRSLAEFGLSEDKKTVLIFGGSRGA-APINRAVIDMQ-DVLKTRDYQVLYITGEVHYEKVMNELKSKGAADNMVTKPFLHQMPEYLKAIDVIVARAGATTIAEITALGIPSVLIPSPYVTANHQEVNARSLGQHDAAIVLKETELSGEKLIEALDRIVLNEQTLKEMSERTKSLGVPDAAARLYSVLEELKK 4RIF Chain:A ((1-372)) MKILFVASGSPATVFALAPLATAAR-NA-GHDVFMGAVEDM-VPYIASA-GIPAL--SIAPSSIRRYATMDREG--EEELDFAGHWFGRMAAGSMDALREVTANWRPDLVVGGSMSF--AAALIAAELGVPYVRQAWDTGDAWRTDPAASDELRPELRALGLDRLPDPALFVDICPPSLRPATAPPAQMMRWVPANGQRRLEP--------WMYTKGNRPRILVTSGSRLVFAKKTG-FLRGLVADMAALDAE-VVIATLDEVAEELR-----TELPGVR-AGWV-PLDVVVPTCDVVVHHAGGVTALTAMNAGVPQLIVPQGG----NFVEAGLRISDFGAAITVDENTPE--AVEKACGELIGNPSYAERARELSAEIAALPLPAEVVGALEGL--

General information: TITO was launched using: RESULT: Template: 4RIF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1920 -213749 -111.33 -651.67 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -111.33 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_4RIF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RIF-query.scw PDB file : Tito_Scwrl_4RIF.pdb: