Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKFPKKLLPIAVLSSIAFSSLASGSVPEASAQEKKKGNQDEIKNVIVLIGDGMGVSYTSAYRYLKDNKKTK---V--VEPTAFDQYLVGQQ-TTYPDDPEQNVTDSAAAATAMSAGIKTYNNAIAVDNDGSEAKTVLEAAKEKGKATGLVATSEITHATPASFGSHDHSRKNMNSIADDYFDEMVNGKHKIDVLLGGGKSNFDRKD----------------------------RNLIKEFKKAGYSYVDDRKDMLKNKDSQVLGLFADGGLPKKIDRT-------KDIPSLKDMTNTAIKKLNKDKDGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFEQAYKAAIDFAKKDKHTLVVATADHSTGGYSIGADGI-----------------Y--NW-----F-SEPIKAAKRTPDFMAEKIADG-------ADVEKTLKTYIDQKKLALTKAEIQSVEEAA------------------------KSKEVLDIDNAIENIFNKRSHTGWTTGGHTGEDVPVYAYGPSSE--TFAGQIDNTEIAKNVFKALQYNIKINDK 3E2D Chain:A ((2-502)) -----------------------------------------EIKNVILMIGDGMGPQQVGLLETYANQAPNSIYKGNKTAIYQLAQEGVIGSSLTHPE--DAIVVDSACSATMLATGIYSSSEVIGIDSQGNHVETVLEKAKKAGKATGLVSDTRLTHATPASFAAHQPHRSLENQIASDMLAT------GADVMLSGGLRHWIPKSTNDKGETYKQLEKLTQGDVYLKSKRKDDRNLLTEAEKDGYQLAFNRNMLDDAKGDKLLGLFAYSGMDDGIAYSNKKKSGERTQPSLKEMTQKALNILSKDEDGFFLMVEGGQIDWAGHSNDAGTMLHELLKFDEAIQTVYEWAKDREDTIVIVTADHETGSFGFSYSSNDLPKPQKRSGEAFADRDYAPNFNFGAFDILDGLYNQKQSYYGMISEFQKLDKSLQTPEKLAEIVNKNSE---FPITAEQAKNVLASKPNPYRLAQHKYLSAEEVPAINDFDAFFPYNDRGNLLAREQATGQNIVWGTGTHTHTPVNVFAWGPAEKILPVSKIMHHSELGEYIKQQVN--------

General information: TITO was launched using: RESULT: Template: 3E2D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2590 -137690 -53.16 -342.51 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -53.16 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_3E2D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E2D-query.scw PDB file : Tito_Scwrl_3E2D.pdb: