Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEEKRSSTGFLVKQRAFLKLYMITMTEQERLYGLKLLEVLRSEFKEIGFKPNHTEVYRSLHELLDDGILKQIKVKKEGAKLQEVVLYQFKD--YEAAKLYKKQLKVELDRCKKLIEKALSDNF 2ESH Chain:A ((11-110)) ---------------GWWLASTILLLVAEKPSHGYELAERLAEFGIEIPGIGHMGNIYRVLADLEESGFLSTEWDT---TVSPPRKIYRITPQGKLYLREILRSLEDMKRRIETLEER------

General information: TITO was launched using: RESULT: Template: 2ESH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 293 -41567 -141.87 -424.15 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -141.87 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.639

(partial model without unconserved sides chains): PDB file : Tito_2ESH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ESH-query.scw PDB file : Tito_Scwrl_2ESH.pdb: