Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIVKATDQSFSAETSEGVVLADFWAPWCGPCKMIAPVLEELDQEMGDKLKIVKIDVDENQETAGKYGVMSIPTLLVLKDGEVVETSVGFKPKEALQELVNKHL 3TCO Chain:B ((20-108)) ---------------NNKLVLVDCWAEWCAPCHLYEPIYKKVAEKYKGKAVFGRLNVDENQKIADKYSVLNIPTTLIFVNGQLVDSLVGAVDEDTLESTVNKYL

General information: TITO was launched using: RESULT: Template: 3TCO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 357 -66613 -186.59 -748.46 target 2D structure prediction score : 0.82 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -186.59 2D Compatibility (Sec. Struct. Predict.) : 0.82 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.804

(partial model without unconserved sides chains): PDB file : Tito_3TCO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TCO-query.scw PDB file : Tito_Scwrl_3TCO.pdb: