Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTMKQFEIAAIPGDGVGKEVVAAAEKVLHTAAEVHGGLSFSFTAFPW-SCDYYLEHGKMMPEDGIHTLTQFEAVFLGAVGNPKLVPDHISLWGLLLKIRRELELSINMRPAKQMAGITSPLLH--PNDFDFVVIRENSEGEYSEVGGRIHRG-DDEIAIQNAVFTRKATERVMRFAFELAKKRR-SHVTSATKSNGIYHAMPFWDEVFQQTAADYSGIETSSQHIDALAAFFVTRPETFDVIVASNLFGDILTDISSSLMGSIGIAPSANINPSGKYPSMFEPVHGSAPDIAGQGLANPIGQIWTAKLMLDHFGEEE-----LGAKILDVMEQVTADGIKTRDIGGQSTTAEVTDEICSRLRKL 3FLK Chain:A ((5-363)) ----SFRIAAIPGDGIGLEVLPEGIRVLEAAALKH-GLALEFDTFEWASCDYYLQHGKMMPDDWAEQLKQYDAIYFGAVGWPDKVPDHISLWGSLLKFRREFDQYVNIRPVRLFPGVPCALANRKVGDIDFVVVRENTEGEYSSLGGIMFENTENEIVIQESIFTRRGVDRILKYAFDLAEKRERKHVTSATKSNGMAISMPYWDKRTEAMAAHYPHVSWDKQHIDILCARFVLQPERFDVVVASNLFGDILSDLGPACAGTIGIAPSANLNPERNFPSLFEPVHGSAPDIFGKNIANPIAMIWSGALMLEFLGQGDERYQRAHDDMLNAIERVIADGSVTPDMGGTLSTQQVGAAISDTLARL

General information: TITO was launched using: RESULT: Template: 3FLK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1999 -187518 -93.81 -537.30 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -93.81 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.528

(partial model without unconserved sides chains): PDB file : Tito_3FLK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FLK-query.scw PDB file : Tito_Scwrl_3FLK.pdb: