Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYTVTLNPSVDYIVHVEDFTVGGLNRSSYDTKYPGGKGINVSRLLKRHHVASKALGFVGGFTGEYIKTFLREENLETAFSEVKGDTRINVKLKT-GDETEINGQGPTISDEDFKAFLEQF-QSLQEGDIVVLAGSIPSSLPHDTYEKIAEACKQQNARVVLDISGEALLKATE--MKPFLMKPNHHELGEMFGTAI-TSV-EEAVPYGKKLVEQGAEHVIVSMAGDGALLFTNEAVYFANVPKGKLVNSVGAGDSVVAGFLAGISKQLPLEEAFRLGVTSGSATAFSEELG--TEEFVQQLLPEVKVTRL 2F02 Chain:A ((3-311)) LIVTVTMNPSIDISYLLDHLKLDTVNRTSQVTKTPGGKGLNVTRVIHDLGGDVIATGVLGGFHGAFIANELKKANIPQAFTSIKEETRDSIAILHEGNQTEILEAGPTVSPEEISNFLENFDQLIKQAEIVTISGSLAKGLPSDFYQELVQKAHAQEVKVLLDTSGDSLRQVLQGPWKPYLIKPNLEELEGLLGQDFSENPLAAVQTALTKPMFAGIEWIVISLGKDGAIAKHHDQFYRVKIPTIQAKNPVGSGDATIAGLAYGLAKDAPAAELLKWGMAAGMANAQERMTGHVDVENVKKHLMNIQVV--

General information: TITO was launched using: RESULT: Template: 2F02.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1690 -146333 -86.59 -486.16 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -86.59 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_2F02.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F02-query.scw PDB file : Tito_Scwrl_2F02.pdb: