Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQFDKQYNSIIKDIINNGISDEEFDVRTKWDSDGTPAHTLSVMSKQMRFDNS-EVPILTTKKVAWKTAIKELLWIWQLKSNDVNDLNKMGVHIWDQWKQE------------DGTIGHAYGFQLGKKNRSLN---------------------------------GEKVDQVDYLLHQLKNNPSSRRHITMLWNPDDLDAMSLTPCVYETQWYVKH--GKLHLEVRARSNDMALGNPFNVFQYNVLQRMIAQVTGYELGEYIFNIGDCHVYTRHIDNLKIQMEREQFEAPELWINPEV-----------KDFYNFTIDDFKLINYKHGDKLLFEVAV 3V8H Chain:A ((5-318)) ----MKQYLDLVRTILDTGTWQS--N---GI-----R--TIGIPGAMLRFDLQQGFPAVTTKKLAFKSAIGELVGFLRATR-SAAEFRALGCKVWDANANENAQWLANPYRRGADDLGDVYGVQWRRWPGYKVLDAHADAQIADATSRGFRIVARFEEGGADKVLLHKAIDQLRDCLDTIVRDPSSRRILFHGWNPAVLDEIALPACHLLYQFLPNVERREISLCLYIRSNDVGLGTPFNLAEGAALLTLVGRLTGYSPRWFTYFIGDAHIYENQLDMLKQQLEREPFESPRLELAERVPDYAKTGKYEPQWLERVEPSDFTLVGYRHH---------

General information: TITO was launched using: RESULT: Template: 3V8H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1164 -8264 -7.10 -32.66 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -7.10 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.545

(partial model without unconserved sides chains): PDB file : Tito_3V8H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3V8H-query.scw PDB file : Tito_Scwrl_3V8H.pdb: