Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVKVAINGFGRIGRMVFRKAMLDDQIQVVAINASYSAETLAHLIKYDTIHGRYDKEVVAGEDSLIVNGKKVLLLNSRDPKQLPWREYDIDIVVEATGKFNAKDKAMGHIEAGAKKVILTAPGKNEDVTIVMGVNEDQFDAERHVIISNASCTTNCLAPVVKVLDEEFGIESGLMTTVHAYTNDQKNIDNPH-KDLRRARACGESIIPTTTGAAKALSLVLPHLKGKLHGLALRVPVPNVSLVDLVVDLKTDVTAEEVNEAFKRAAKTSMYGVLDYSDEPLVSTDYNTNPHSAVIDGLTTMVMEDRKVKVLAWYDNEWGYSCRVVDLIRHVAARMKHPSAV 4LSM Chain:A ((16-346)) -TIKIGINGFGRIGRMVFRAAQHRNDIEIVGINDLLDADYMAYMLKYDSTHGRYGGMVEVREGALVVNGKKIRVTSERDPANLKWNEVGAVVVVESTGLFLTDETARKHIQAGAKKVVMTGPPKDDTPMFVMGVNNTMYKG--QEIVSNASCTTNCLAPLAKIIHEKFGIVEGLMTTVHATTATQKTVDGPSQKDWRGGRGAAQNIIPSATGAAKAVGKVIPALNGRLTGMAFRVPTPNVSVVDLTARLERPATYEQICAAIKAASEGELKGILGYTEDEVVSTDMNGVALTSVFDVKAGISLNDRFVKLISWYDNETGYSHKVLDLVAYISAH-------

General information: TITO was launched using: RESULT: Template: 4LSM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1966 -128568 -65.40 -389.60 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -65.40 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.601

(partial model without unconserved sides chains): PDB file : Tito_4LSM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LSM-query.scw PDB file : Tito_Scwrl_4LSM.pdb: