Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHTVTQTSLYGKDLLTLKDLSEEDINALLAEAGELKQNK----IQPIFHGKTLAMIFEKSSTRTRVSFEAGMAQLGGSALFLSQKDLQLGRGETVADTAKVLSGYVDAIMIRTFEHEKVEELAKEADIPVINGLTDKYHPCQALADLLTIKEIKG--KLKGVKVAYIGD-GNNVAHSLMIGCAKMGCDISIASPKGYEVLDEAAEAAKTYALQSGSSVTLTDDPIEAVKDADVIYSDVFTSMGQEAEE-QERLAVFAPYQVNAALVSH-AKPDYTFLHCLPAH------------------REEEVTAEIIDGPNSAVFQQAENRLHVQKALLKAILYKGESSKNC 1DUV Chain:G ((2-333)) -------GFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLTGKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYDGIQYRGYGQEIVETLAEYASVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLVYAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTEDVAKGVEGADFIYTDVWVSMGEAKEKWAERIALLREYQVNSKMMQLTGNPEVKFLHCLPAFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK-------

General information: TITO was launched using: RESULT: Template: 1DUV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 1828 -167971 -91.89 -550.72 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain G : 0.80 3D Compatibility (PKB) : -91.89 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.549

(partial model without unconserved sides chains): PDB file : Tito_1DUV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DUV-query.scw PDB file : Tito_Scwrl_1DUV.pdb: