Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISFIFAMDANRLIGKDNDLPWHLPNDLAYFKKITSGHSIIMGRKTFESIG-RPLPNRKNIVVTSAPDSEFQGCTVVSSLKDVLDICSGPEECFVIGGAQ-LYTDLFPYADRLYMTKIHHEFEGDRHFPEFDESNWKLVSSEQGT-KDEKNPYDYEFLMYEKKNSSKAGGF 4M7U Chain:A ((9-170)) MLAAIWAQDEQGVIGKEGKLPWHLPNDLKFFKEKTIHNTLVLGRATFEGMGCRPLPNRTTIVLTSNPDYQAEGVLVMHSVEEILAYADKYEGVTVIGGGSVVFKELIPACDVLYRTMIHETFEGDTFFPEIDWSVWEKVA-TVPGVVDEKNLYAHDYETYHRN--------

General information: TITO was launched using: RESULT: Template: 4M7U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 762 -31508 -41.35 -198.16 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -41.35 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.631

(partial model without unconserved sides chains): PDB file : Tito_4M7U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M7U-query.scw PDB file : Tito_Scwrl_4M7U.pdb: