Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQFDKQYNSIIKDIINNGISDEEFDVRTKWDSDGTPAHTLSVMSKQMRFDNS-EVPILTTKKVAWKTAIKELLWIWQLKSNDVNDLNKMGVHIWDQWKQEDGTIGHAYGFQLGKKNRSLNGEKVDQVDYLLHQLKNNPSSRRHITMLWNPDDLDAMSLTPCVYETQWYVKHGKLHLEVRARSNDMALGNPFNVFQYNVLQRMIAQVTGYELGEYIFNIGDCHVYTRHIDNLKIQMEREQFEAPELWINPEVKDFYNFTIDDFKLINYKHGDKLLFEVAV 4FQS Chain:A ((1-263)) ----MTPYEDLLRFVLETGTPKS--DRT-GT-----G--TRSLFGQQMRYDLSAGFPLLTTKKVHFKSVAYELLWFLRGDS-NIGWLHEHGVTIWDEWASDTGELGPIYGVQWRSWPAPS-GEHIDQISAALDLLRTDPDSRRIIVSAWNVGEIERMALPPCHAFFQFYVADGRLSCQLYQRSADLFLGVPFNIASYALLTHMMAAQAGLSVGEFIWTGGDCHIYDNHVEQVRLQLSREPRPYPKLLLAD-RDSIFEYTYEDIVVKNYDPHPAIKAPVAV

General information: TITO was launched using: RESULT: Template: 4FQS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1335 -33952 -25.43 -129.59 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -25.43 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.416

(partial model without unconserved sides chains): PDB file : Tito_4FQS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FQS-query.scw PDB file : Tito_Scwrl_4FQS.pdb: