Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLEEKTIAKEQIFSGKVIDLYVEDVELPNGKASKREIVKHPGAVAVLAVTDEGKIIMVKQFRKPLERTIVEIPAGKLEKGEEPEYTALRELEEETGYTAKKLTKITAFYTSPGFADEIVHVFLAEELSVLEEKRELDEDEFVEVMEVTLEDALKLVESREVYDAKT-AYAIQYLQLKEALQAQK 1V8Y Chain:A ((11-168)) ----------RTYLYRGRILNLALE---------GRYEIVEHKPAVAVIALR-EGRMLFVRQMRPAVGLAPLEIPAGLIEPGEDPLEAARRELAEQTGLSGDLTYLFS-YFVSPGFTDEKTHVFLAENLKEV--E--------IEVVWMRPEEALERHQRGEVEFSATGLVGVLYYHAFLR-----

General information: TITO was launched using: RESULT: Template: 1V8Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 690 -77174 -111.85 -517.95 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -111.85 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_1V8Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V8Y-query.scw PDB file : Tito_Scwrl_1V8Y.pdb: